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Free Radicals And Antioxidant Protocols: Preliminary Entry 2034 (Methods In Molecular Biology)
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Free Radicals And Antioxidant Protocols: Preliminary Entry 2034 (Methods In Molecular Biology)

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Description

In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Prools, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include prools to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and user-friendly, Free Radicals and Antioxidant Prools, Second Edition, is an essential guide for the novice and expert researcher alike.


Table Of Contents:

Preface v

Contributors xi

Section I Reactive Oxygen and Nitrogen Species 1

1 In Vivo Imaging of Free Radicals and Oxygen Deepti S. Vikram Brian K. Rivera Periannan Kuppusamy 3

2 In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle, Lithium Phthalocyanine F. Hyodo S. Matsumoto E. Hyodo A. Matmmoto K. Matsumoto M.C. Krishna 29

3 Measurement of Plasma Nitrite by Chemiluminescence Enika Nagababu Joseph M. Rifkind 41

4 Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyobiasts Exposed to Reactive Oxygen and Nitrogen Species K. Sathishkumar Xueli Gao Achuthan C. Raghavamenon Subramanyam N. Murthy Philip J. Kadowitz Rao M. Uppu 51

Section II Natural and Synthetic Antioxidants 63

5 Phenolic Acids and Flavonoids: Occurrence and Analytical Methods Constantine D. Stalikas 65

6 Design, Synthesis, and Action of Antiatherogenic Antioxidants Osama Oynshi Kunio Tamura Etsuo Niki 91

7 Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity Against Lipid Peroxidation Adriana R Pohlmann Scheila Rezende Schaffazick T?nia B. Creczynski-Pasa S?lvia S. Guterres 109

8 Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy Gang Liu Ping Men George Perry Mark A. Smith 123

9 Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes Eric Simone Thomas Dziubla Vladimir Shavaev Vladimir R. Muzykantov 145

10 Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo Enika Nagababu Joseph M. Rifkind Sesikeran Boindala Lakshmaiah Nakka 165

Section III Cellular Oxidative Stress 181

11 The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia Douglas R. Spitz Shannon J. Sullivan 183

12 A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling Michelle A. Kline E.S. O'Connor Butler Adam Hinzey Sean Sliman Sainath R. Kotha Clay B. Marsh Rao M. Uppu Narasimham L. Parinandi 201

13 Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction W. Deng T.J. Bivalacqua H.C. Champion W.J. Hellstrom Subramanyam N. Murthy Philip J. Kadowitz 213

14 Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids James M. May 229

15 Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening Muthuswamy Balasubramanyam Antonysunil Adaikalakoteswari Zaheer Sameermahmood Viswanathan Mohan 245

16 Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic Neurons Exposed to Cholesterol Secoaldehyde K. Sathishkumar Achuthan C. Raghavamenon Karunakaran Gctneshkumar Rameshwari Telaprolu Narasimham L. Parinandi Rao M. Uppu 263

17 Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration Vittorio Calabrese Carotin Cornelius Cesare Mancuso Riccardo Lentile A.M. Giujfrida Stella D. Allan Butterfield 285

18 Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension W. Deng T.J. Bivalacqua H.C. Champion W.J. Hellstrom Subramanyam N. Murthy Philip J. Kadowitz 309

Section IV DNA Oxidation, Oxidative Lipidomics, and Biomarkers 323

19 A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA Yelena Margolin Peter C. Dedon 325

20 Analysis of Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine by Liquid Chromatography-Tandem Mass Spectrometry Mark D. Evans Rajinder Singh Vitas Mistry Peter B. Farmer Marcus S. Cooke 341

21 Oxidative Lipidomics of Apoptosis: Quantitative Assessment of Phospholipid Hydroperoxides in Cells and Tissues Vladimir A. Tyurin Yulia Y. Tyurina Vladimir B. Ritov Andriy Lysytsya Andrew A. Amoscato Patrick M. Kochanek Ronald Hamilton Steven T. DeKosky Joel S. Greenberger H?lya Bayir Valerian E. Kagan 353

22 Simultaneous Analysis of Multiple Lipid Oxidation Products In Vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS) Huiyong Yin Todd Davis Ned A. Porter 375

23 Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides E. Susan O'Connor Butler Jessica N. Mazerik Jason P. Cruff Shariq I. Sherwani Barbara K. Weis Clay B. Marsh|cAchuthan C. Raghavamenon Rao M. Uppu Harald H. O. Schmid Narasimham L. Parinandi 387

24 Oxidized Low-Density Lipoprotein Sampath Parthasarathy Achuthan Raghavamenon Mahdi Omar Garelnabi Nalini Santanam 403

25 Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease Paula I. Moreira Lawrence M. Sayre Xiongwei Zhu Akihiko Nunomura Mark A. Smith George Perry 419

26 Enzyme Immunoassay of Isoprostanes Denis M. Callewaert Charles Sloan 435

27 Application of Membrane Extraction with Sorbent Interface for Breath Analysis Victor Ma Heather Lord Melissa Morley Janusz Pawliszyn 451

Subject Index 469

Details

Book: Free Radicals And Antioxidant Protocols: Preliminary Entry 2034 (Methods In Molecular Biology)
Author: , , ,
ISBN-13: 9781588297105
Product Code 9781588297105
Binding: Hard Bound
Publishing Date: 2009-12-01
Publisher: Humana Press
Number of Pages: 496
Language: English

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